Genetic diversity and population structure of the endangered marsupial Sarcophilus harrisii (Tasmanian devil).

نویسندگان

  • Webb Miller
  • Vanessa M Hayes
  • Aakrosh Ratan
  • Desiree C Petersen
  • Nicola E Wittekindt
  • Jason Miller
  • Brian Walenz
  • James Knight
  • Ji Qi
  • Fangqing Zhao
  • Qingyu Wang
  • Oscar C Bedoya-Reina
  • Neerja Katiyar
  • Lynn P Tomsho
  • Lindsay McClellan Kasson
  • Rae-Anne Hardie
  • Paula Woodbridge
  • Elizabeth A Tindall
  • Mads Frost Bertelsen
  • Dale Dixon
  • Stephen Pyecroft
  • Kristofer M Helgen
  • Arthur M Lesk
  • Thomas H Pringle
  • Nick Patterson
  • Yu Zhang
  • Alexandre Kreiss
  • Gregory M Woods
  • Menna E Jones
  • Stephan C Schuster
چکیده

The Tasmanian devil (Sarcophilus harrisii) is threatened with extinction because of a contagious cancer known as Devil Facial Tumor Disease. The inability to mount an immune response and to reject these tumors might be caused by a lack of genetic diversity within a dwindling population. Here we report a whole-genome analysis of two animals originating from extreme northwest and southeast Tasmania, the maximal geographic spread, together with the genome from a tumor taken from one of them. A 3.3-Gb de novo assembly of the sequence data from two complementary next-generation sequencing platforms was used to identify 1 million polymorphic genomic positions, roughly one-quarter of the number observed between two genetically distant human genomes. Analysis of 14 complete mitochondrial genomes from current and museum specimens, as well as mitochondrial and nuclear SNP markers in 175 animals, suggests that the observed low genetic diversity in today's population preceded the Devil Facial Tumor Disease disease outbreak by at least 100 y. Using a genetically characterized breeding stock based on the genome sequence will enable preservation of the extant genetic diversity in future Tasmanian devil populations.

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منابع مشابه

Allorecognition in the Tasmanian Devil (Sarcophilus harrisii), an Endangered Marsupial Species with Limited Genetic Diversity

Tasmanian devils (Sarcophilus harrisii) are on the verge of extinction due to a transmissible cancer, devil facial tumour disease (DFTD). This tumour is an allograft that is transmitted between individuals without immune recognition of the tumour cells. The mechanism to explain this lack of immune recognition and acceptance is not well understood. It has been hypothesized that lack of genetic d...

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The Tasmanian devil (Sarcophilus harrisii) is a carnivorous marsupial found only in the wild in Tasmania, Australia. Tasmanian devils are classified as endangered and are currently threatened by devil facial tumour disease, a lethal transmissible cancer that has decimated the wild population in Tasmania. To prevent extinction of Tasmanian devils, conservation management was implemented in 2003 ...

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Extensive population decline in the Tasmanian devil predates European settlement and devil facial tumour disease.

The Tasmanian devil (Sarcophilus harrisii) was widespread in Australia during the Late Pleistocene but is now endemic to the island of Tasmania. Low genetic diversity combined with the spread of devil facial tumour disease have raised concerns for the species' long-term survival. Here, we investigate the origin of low genetic diversity by inferring the species' demographic history using tempora...

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Transmission of a fatal clonal tumor by biting occurs due to depleted MHC diversity in a threatened carnivorous marsupial.

A fatal transmissible tumor spread between individuals by biting has emerged in the Tasmanian devil (Sarcophilus harrisii), a carnivorous marsupial. Here we provide genetic evidence establishing that the tumor is clonal and therefore foreign to host devils. Thus, the disease is highly unusual because it is not just a tumor but also a tissue graft, passed between individuals without invoking an ...

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Low major histocompatibility complex diversity in the Tasmanian devil predates European settlement and may explain susceptibility to disease epidemics.

The Tasmanian devil (Sarcophilus harrisii) is at risk of extinction owing to the emergence of a contagious cancer known as devil facial tumour disease (DFTD). The emergence and spread of DFTD has been linked to low genetic diversity in the major histocompatibility complex (MHC). We examined MHC diversity in historical and ancient devils to determine whether loss of diversity is recent or predat...

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عنوان ژورنال:
  • Proceedings of the National Academy of Sciences of the United States of America

دوره 108 30  شماره 

صفحات  -

تاریخ انتشار 2011